Fusicoccin Counteracts the Toxic Effect of Cadmium on the Growth of Maize Coleoptile Segments

The effects of cadmium (Cd; 0.1-1000 μM) and fusicoccin (FC) on growth, Cd(2+) content, and membrane potential (E(m)) in maize coleoptile segments were studied. In addition, the E(m) changes and accumulation of Cd and calcium (Ca) in coleoptile segments treated with Cd(2+) combined with 1 μM FC or 30 mM tetraethylammonium (TEA) chloride (K(+)-channel blocker) were also determined. In this study, the effects of Ca(2+)-channel blockers [lanthanum (La) and verapamil (Ver)] on growth and content of Cd(2+) and Ca(2+) in coleoptile segments were also investigated. It was found that Cd at high concentrations (100 and 1000 μM) significantly inhibited endogenous growth of coleoptile segments and simultaneously measured proton extrusion. FC combined with Cd(2+) counteracted the toxic effect of Cd(2+) on endogenous growth and significantly decreased Cd(2+) content (not the case for Cd(2+) at the highest concentration) in coleoptile segments. Addition of Cd to the control medium caused depolarization of E (m), the extent of which was dependent on Cd concentration and time of treatment with Cd(2+). Hyperpolarization of E(m) induced by FC was suppressed in the presence of Cd(2+) at 1000 μM but not Cd(2+) at 100 μM. It was also found that treatment of maize coleoptile segments with 30 mM TEA chloride caused hyperpolarization of E (m) and decreased Cd(2+) content in coleoptile segments, suggesting that, in the same way as for FC, accumulation of Cd(2+) was dependent on plasma membrane (PM) hyperpolarization. Similar to FC, TEA chloride also decreased Ca(2+) content in coleoptile segments. La and Ver combined with Cd(2+) (100 μM) significantly decreased Cd content in maize coleoptile segments, but only La completely abolished the toxic effect of Cd(2+) on endogenous growth and growth in the presence of FC. Taken together, these results suggest that the mechanism by which FC counteracts the toxic effect of Cd(2+) (except at 1000 μM Cd(2+)) on the growth of maize coleoptile segments involves both stimulation of PM H(+)-ATPase activity by FC as well as Cd(2+)-permeable, voltage-dependent Ca channels, which are blocked by FC and TEA chloride-induced PM hyperpolarization.